Role of fibrosis-related genes and pancreatic duct obstruction in rat pancreatitis models: implications for chronic pancreatitis.
نویسندگان
چکیده
Human chronic pancreatitis is characterized by irreversible fibrosis, whereas pancreatic fibrosis in animal models is reversible. In this study, we compare the development of pancreatic fibrosis in the dibutyltin dichloride (DBTC) model, WBN/Kob rats and bile duct-ligated (BDL) rats. DBTC (8 mg/kg) was administered to LEW rats, and the pancreas was histopathologically investigated sequentially. Male and female WBN/Kob rats aged 4, 6 and 8 months were also examined. BDL rats were prepared by ligation of the bile duct at the duodenal portion and sacrificed at 3 or 7 days after ligation. Fibrosis in the DBTC model peaked after 1 week and was limited to the areas around the pancreatic ducts after 2 weeks, and was composed of both type I and type III collagen. In contrast, fibrosis in male WBN/Kob rats peaked at age 4 months, expanded into intralobular area, and was composed of type III collagen. It exhibited almost no type I collagen and a marked tendency to regress. Pancreatic fibrosis in BDL rats was somewhat difficult to induce and required increased stimulation. This suggests that fibrosis in human biliary pancreatitis may gradually form based on weak, continuous stimulation. We conclude that type I collagen may be involved in the progression of irreversible fibrosis. The imbalance between synthesis and degradation of extracellular matrix molecules or degree of stimulation over a certain period may lead to pancreatic fibrosis. Gene expressions of prolyl hydroxylase and tissue inhibitors of matrix metalloproteinase-2 were elevated.
منابع مشابه
A new animal model for local pancreatitis, ventral pancreatic duct ligation in the adult house musk shrew, Suncus murinus
Various animal models for pancreatitis, using large or small animal models, have been established to study pathophysiology, structure, diagnosis, and treatment of pancreatic disorders. Obstruction of the pancreatic duct is considered to play an important role in the progression of acute and chronic pancreatitis in humans. For this reason, many studies using pancreatic duct ligation animals have...
متن کاملClinical Approach to Chronic Pancreatitis
Chronic pancreatitis leads to significant morbidity and health care costs, in large part due to the pain that it produces. Investigations have found that chronic pancreatitis leads to significant impairments in health-related quality of life [1]. The most notable impairment is in the ability to perform usual daily activities due to compromises in physical and emotional well-being. This was foun...
متن کاملHereditary chronic pancreatitis
Hereditary chronic pancreatitis (HCP) is a very rare form of early onset chronic pancreatitis. With the exception of the young age at diagnosis and a slower progression, the clinical course, morphological features and laboratory findings of HCP do not differ from those of patients with alcoholic chronic pancreatitis. As well, diagnostic criteria and treatment of HCP resemble that of chronic pan...
متن کاملNon-alcoholic duct destructive chronic pancreatitis.
BACKGROUND The pathology of non-alcoholic chronic pancreatitis has not yet been sufficiently studied. AIMS To identify the major changes of pancreatic tissue in patients surgically treated for non-alcoholic chronic pancreatitis. PATIENTS Pancreatectomy specimens from 12 patients with non-alcoholic chronic pancreatitis, including four patients with autoimmune or related diseases (Sjögren's s...
متن کاملExpression of interleukin-8 in human obstructive pancreatitis.
CONTEXT Obstructive pancreatitis is a specific form of pancreatitis, which is caused by the obstruction of the main pancreatic duct due to tumors or some other causes. Interleukin-8 is induced in acute pancreatitis, but its expression in obstructive pancreatitis has not been clarified. OBJECTIVE We attempted to provide some insight into the significance of interleukin -8 in the pathogenesis o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Histology and histopathology
دوره 22 10 شماره
صفحات -
تاریخ انتشار 2007